| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127698 |
| Chromosome: | chromosome 13 |
| Location: | 942536 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568100 | (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGTCTACGAGTAGAGCAAGGCACTGG |
| Internal bar code: | CTGGCCCGTCATGGGCTCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 99.7748 |
| LEAP-Seq n confirming: | 3987 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCCACACACACGCTATC |
| Suggested primer 2: | AGCGTGTGGACACTAATCCC |