| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127699 |
| Chromosome: | chromosome 10 |
| Location: | 3423746 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444550 | SPP1A,SPPA1,SPP1 | Signal peptide peptidase; (1 of 1) K04773 - protease IV [EC:3.4.21.-] (sppA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCACTGATGGCAATAGGCACTCAAGTC |
| Internal bar code: | TAAAGATTGCAAGCAGCATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 99.4366 |
| LEAP-Seq n confirming: | 353 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAACCGCTGTCTGCCTCT |
| Suggested primer 2: | AGTGGGATTGAGGTCAGGTG |