| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127702 |
| Chromosome: | chromosome 7 |
| Location: | 2706778 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331100 | PHC11 | Putative pherophorin-chlamydomonas homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTGCCCGCGCCCAGTCCTCTCGCGCCG |
| Internal bar code: | TCAACACAGTTGACCCGGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 68.4685 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATCTGGTGATGTCCGAA |
| Suggested primer 2: | ATGGGCATGAATCCAATGAT |