| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127706 |
| Chromosome: | chromosome 17 |
| Location: | 765233 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701500 | DNJ1 | (1 of 1) K09503 - DnaJ homolog subfamily A member 2 (DNAJA2); DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAATGCGCGAGGGAGGCATATGTAAATCG |
| Internal bar code: | CAAGTTTTTAGATCAAAGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 99.5575 |
| LEAP-Seq n confirming: | 2025 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAATGACGAGCTTGACGA |
| Suggested primer 2: | TAGTCAAGGGGTCAACAGGG |