| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127706 |
| Chromosome: | chromosome 17 |
| Location: | 765238 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g701500 | DNJ1 | (1 of 1) K09503 - DnaJ homolog subfamily A member 2 (DNAJA2); DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTAGCTGCCTCCATGGCTTCCCACAGT |
| Internal bar code: | CAACGGAATTAGGCAGGCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 97.9839 |
| LEAP-Seq n confirming: | 1944 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAATGACGAGCTTGACGA |
| Suggested primer 2: | TAGTCAAGGGGTCAACAGGG |