Insertion junction: LMJ.RY0402.127740_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.127740
Chromosome:chromosome 2
Location:221794
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g074720 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGCTGGATCTTTAACTACCTGGTATTGG
Internal bar code:AAATAGATCGCGAGCAGATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTAAGTTTCCCGGCGTT
Suggested primer 2:GGTGTTGTGCGTGAAGAATG