| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127774 |
| Chromosome: | chromosome 6 |
| Location: | 2618162 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269752 | TRXm,TRXM1,TRX15 | (1 of 1) IPR000104//IPR005746//IPR012336//IPR013766 - Antifreeze protein, type I // Thioredoxin // Thioredoxin-like fold // Thioredoxin domain; Thioredoxin m, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGCAAGGAGTTTGTCAGCGTTTGCAAC |
| Internal bar code: | CATGTTGTGGGCGCTCCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 99.8411 |
| LEAP-Seq n confirming: | 1885 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGACAAGCCTCTTAGCGT |
| Suggested primer 2: | CACCTCCACCCTTGCTGTAT |