| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127775 |
| Chromosome: | chromosome 16 |
| Location: | 6407853 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673700 | (1 of 1) K14799 - pre-rRNA-processing protein TSR1 (TSR1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTAACTCTGCAACCCACTCTGCAACCCC |
| Internal bar code: | AACCTGGTGCCAAGGTGAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 99.486 |
| LEAP-Seq n confirming: | 1742 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCGTGACCACTACCGTTG |
| Suggested primer 2: | GGATGTTTGGAAAAGCGGTA |