Insertion junction: LMJ.RY0402.127777_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.127777
Chromosome:chromosome 2
Location:4802743
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g102850 CPZ (1 of 1) IPR000834//IPR016024 - Peptidase M14, carboxypeptidase A // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAACTCACCACATCCTGAGCCGAACACGC
Internal bar code:TGGACCAGTACTAGGCGGGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:656
LEAP-Seq percent confirming:99.3108
LEAP-Seq n confirming:3314
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCAGTTGCAGGAGGAAAC
Suggested primer 2:TCGGTGAACAGAGATTGCAG