| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127792 |
| Chromosome: | chromosome 8 |
| Location: | 3503756 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375084 | (1 of 1) K12822 - RNA-binding protein 25 (RBM25, S164) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAGACTGCCCTATTTGGCGGCACCATT |
| Internal bar code: | TTACTAGGCGTGGCCGCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 470 |
| LEAP-Seq percent confirming: | 80.8097 |
| LEAP-Seq n confirming: | 276757 |
| LEAP-Seq n nonconfirming: | 65723 |
| LEAP-Seq n unique pos: | 248 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCAGCTTAGGTGGAGTC |
| Suggested primer 2: | AGTGCTCATCGCACCTTCTT |