| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127801 |
| Chromosome: | chromosome 1 |
| Location: | 5243924 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036600 | EXN3 | (1 of 2) K14570 - RNA exonuclease 1 (REX1, REXO1, RNH70); Exoribonuclease II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGAGCTAGATGTGTGATATGGGGTAACTT |
| Internal bar code: | TACGTGATCCTTACGAGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 870 |
| LEAP-Seq percent confirming: | 98.4028 |
| LEAP-Seq n confirming: | 3327 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAAGGAGAGCAAGAAGA |
| Suggested primer 2: | GTGTCGCAGCAAGCGTAATA |