Insertion junction: LMJ.RY0402.127806_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.127806
Chromosome:chromosome 7
Location:3015902
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g333400 MMP23 Matrix metalloproteinase; (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTACTTGCCCCGCGCAACCCGCCAACCCA
Internal bar code:GACGTTTTGCCAATCGAGTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:633
LEAP-Seq percent confirming:92.5761
LEAP-Seq n confirming:1247
LEAP-Seq n nonconfirming:100
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACAGCACACCCACTATG
Suggested primer 2:AGAATGGGGCAGAAACACAC