| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127810 |
| Chromosome: | chromosome 16 |
| Location: | 138637 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695218 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAGTAGGCGGCAGGTCCAGCAGGGCGT |
| Internal bar code: | CAAACAATTAGGTGTTTATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 99.0594 |
| LEAP-Seq n confirming: | 3686 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACTGACGGGACTCTCAA |
| Suggested primer 2: | GGTGTCTTGCCAATGTCCTT |