Insertion junction: LMJ.RY0402.127810_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.127810
Chromosome:chromosome 16
Location:138644
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g695218 (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATAATAGCAAATATTGATACGTGGCAACA
Internal bar code:TCACAAAATAACTGCTCTACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:144
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTCTTGCCAATGTCCTT
Suggested primer 2:CACACTGACGGGACTCTCAA