| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.127820 |
| Chromosome: | chromosome 1 |
| Location: | 897899 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAAGCTGTCCTTCATTACAGAATTCGT |
| Internal bar code: | TCCCCCGGCGCCGAAGAGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 99.9046 |
| LEAP-Seq n confirming: | 2094 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATCAGAACGTGCGAGAA |
| Suggested primer 2: | AAGCTTGGCTTTGACTTCCA |