| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127824 |
| Chromosome: | chromosome 3 |
| Location: | 5871780 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189650 | (1 of 1) K04498 - E1A/CREB-binding protein (EP300, CREBBP, KAT3); Histone acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAGGCCAGAGCAATGACAGAGGGAGGGC |
| Internal bar code: | GGCTAAGGCCGCTGCTAGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 99.9639 |
| LEAP-Seq n confirming: | 5543 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATTGATGGTGCTCTGGTT |
| Suggested primer 2: | ACGAGCGGACAAATTAGTGG |