| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.127854 |
| Chromosome: | chromosome 6 |
| Location: | 916123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255100 | (1 of 4) IPR000533 - Tropomyosin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGACGCTGAGCGCCCATCAACACGAG |
| Internal bar code: | ACCACTTTGGAATCGGGTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 807 |
| LEAP-Seq percent confirming: | 99.7067 |
| LEAP-Seq n confirming: | 1020 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTCTCGTCACAGCATTC |
| Suggested primer 2: | CATACCCACGACCTAATGCC |