Insertion junction: LMJ.RY0402.127879_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.127879
Chromosome:chromosome 12
Location:2568975
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g505350 FAP10,ACA2 Flagellar Associated Protein 10; (1 of 2) PTHR24093:SF254 - PLASMA MEMBRANE CALCIUM-TRANSPORTING ATPASE 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGCGACTACGAGACGCACATGCATGACG
Internal bar code:AGTGCTGGCTCGATCACTTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:387
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:913
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAGAGACAACGGAGTGAGCC
Suggested primer 2:CTGTAACTGCCGACGTGTGT