| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127887 |
| Chromosome: | chromosome 4 |
| Location: | 2630142 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCCATGCCGTTCATTCTTCCTACGAAG |
| Internal bar code: | TACTCGACTGCACGGAGCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 96.0186 |
| LEAP-Seq n confirming: | 3714 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGTGCCTGTGTGGCTTA |
| Suggested primer 2: | GGACAAGTAAGGCGGCATAA |