| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.127893 |
| Chromosome: | chromosome 16 |
| Location: | 578995 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692350 | (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAATGTAGCCCCACCCCGTCTGCAGTAT |
| Internal bar code: | GAGTTGAGTCGGCCCATTTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 798 |
| LEAP-Seq percent confirming: | 94.4229 |
| LEAP-Seq n confirming: | 2438 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACAGCACTAGCAGCAA |
| Suggested primer 2: | ATGCTGTAATGAAGGCCTGG |