Insertion junction: LMJ.RY0402.127930_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.127930
Chromosome:chromosome 17
Location:2706822
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g718000 PHC7,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAAGTATAGCGCCGGGCAAGCGTGGGTG
Internal bar code:TCAGCGGACCGCCACCACGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:640
LEAP-Seq percent confirming:99.6495
LEAP-Seq n confirming:853
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGTGGACTTCCCCAAGTG
Suggested primer 2:AACACCTGATAGCACCCGTC