| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.127958 |
| Chromosome: | chromosome 3 |
| Location: | 4991080 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180250 | IPS1,INO1 | (1 of 1) 5.5.1.4 - Inositol-3-phosphate synthase / Myo-inositol-1-phosphate synthase; Myo-inositol-1-phosphate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGATGTGCGGGCTTGGCTGTAAACTGT |
| Internal bar code: | GATTTTCATGCTTTGTTTTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 98.9153 |
| LEAP-Seq n confirming: | 1459 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTCATCCACAACACCTG |
| Suggested primer 2: | GTACCACAGCCCCAGACCTA |