Insertion junction: LMJ.RY0402.127963_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.127963
Chromosome:chromosome 5
Location:411325
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g232850 COX17 Cytochrome c oxidase assembly protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCTCCGGCTTGGAGCCTGATGCGCCCA
Internal bar code:TGTCAGCGATGATTAGGAGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:32
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACTGTCAGCTCCGCATA
Suggested primer 2:AAGACCTGCTGGGTACGATG