Insertion junction: LMJ.RY0402.128016_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.128016
Chromosome:chromosome 15
Location:1120527
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g639504 (1 of 3) PF13637//PF13920 - Ankyrin repeats (many copies) (Ank_4) // Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACGGGCGCATGCGGCGCCAGGAGGGAGT
Internal bar code:TTCAGCATTGACTAGTCCCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:852
LEAP-Seq percent confirming:95.8923
LEAP-Seq n confirming:1354
LEAP-Seq n nonconfirming:58
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TAAGACAGGTTGTTGCAGCG
Suggested primer 2:AGCACACACATCCGTCACAT