Insertion junction: LMJ.RY0402.128030_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128030
Chromosome:chromosome 7
Location:4529514
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g343600 (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTTGTCCCATATTACAACGATCTCACA
Internal bar code:GTGCCTTCTCTGCGGGCGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:270
LEAP-Seq percent confirming:92.0635
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACATCACTGGACACCGAACA
Suggested primer 2:CGGTAGGTACAGGACTGGGA