| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128030 |
| Chromosome: | chromosome 14 |
| Location: | 3789473 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632501 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGCCCTAGCTCACGCCCGGCGCCACTC |
| Internal bar code: | CCGGGTTTGTCCCCACGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.7543 |
| LEAP-Seq n confirming: | 2030 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTACGACACGGCTAATC |
| Suggested primer 2: | TCGTCTGACACTGAGCAAGG |