Insertion junction: LMJ.RY0402.128049_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.128049
Chromosome:chromosome 3
Location:2639653
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g160900 (1 of 1) PF07714//PF12796//PF13857 - Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGCCGTTCCAACTTGTCAGCTTCTAACC
Internal bar code:CCCTGGTCTGCGCCAGATGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:648
LEAP-Seq percent confirming:99.5444
LEAP-Seq n confirming:437
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAAACTAAACCGAACCAA
Suggested primer 2:CGTCACCGACTTCTGAAACA