| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128079 |
| Chromosome: | chromosome 14 |
| Location: | 1121136 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615250 | TRX18 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCACACTTGTGTACCACTGTCATGAC |
| Internal bar code: | AGGTGGCCCCTGCCGGCTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 97.5281 |
| LEAP-Seq n confirming: | 1736 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGTCTTTGGACGGATG |
| Suggested primer 2: | CCAACCCTTCAACCTCGTAA |