| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128086 |
| Chromosome: | chromosome 1 |
| Location: | 1014181 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005813 | POB31 | Proteome of basal body 31 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGATAGGGCCACGGATTGAGGTCAAAT |
| Internal bar code: | AGTGGAGGCAGCTTACCGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 99.6548 |
| LEAP-Seq n confirming: | 2887 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAGGCGACAGCCAAGCTA |
| Suggested primer 2: | TGGTGCACACAATCCAACTT |