Insertion junction: LMJ.RY0402.128115_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128115
Chromosome:chromosome 9
Location:2067295
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g394050 CPLD19 Zinc finger family protein; (1 of 1) PTHR22883:SF91 - PROTEIN S-ACYLTRANSFERASE 12-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCACCCTCCTTCCCCCCCACCTGCCCCCA
Internal bar code:TAGAAAAATGAATTGCGTACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:824
LEAP-Seq percent confirming:58.8193
LEAP-Seq n confirming:827
LEAP-Seq n nonconfirming:579
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGGGGAGTTAAGAGGGAG
Suggested primer 2:ACTGTTACGGTGATCCTGGC