| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128133 |
| Chromosome: | chromosome 9 |
| Location: | 4541091 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396102 | (1 of 1) K08856 - serine/threonine kinase 16 [EC:2.7.11.1] (STK16) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTTGGGTTTGGCACGTGTTGCGTGTG |
| Internal bar code: | TTTATTATTTTGTTCCGTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 86.1624 |
| LEAP-Seq n confirming: | 467 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAGGTCGCAAATACCAGC |
| Suggested primer 2: | GCCATGAGTACGGGTCTTGT |