| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128139 |
| Chromosome: | chromosome 12 |
| Location: | 1408523 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488050 | FFT5 | putative fructan fructosyltransferase; (1 of 1) 2.4.1.99//3.2.1.26 - Sucrose:sucrose fructosyltransferase / Sucrose:sucrose 1-fructosyltransferase // Beta-fructofuranosidase / Saccharase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGTTGGGCGTCGTCGCGGGGTGTGCAC |
| Internal bar code: | TCAGGCCCGGGATTCCAGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 99.6573 |
| LEAP-Seq n confirming: | 1745 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGACTACTCCTGCCTGG |
| Suggested primer 2: | CAGGAGCGTCTACTTGGGAG |