| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.128186 |
| Chromosome: | chromosome 5 |
| Location: | 1075601 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246150 | CYG13 | Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGGAGGTCTTGATTAAGGATGTGTCAA |
| Internal bar code: | CAGGGCTCCCCACCAACCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 99.6697 |
| LEAP-Seq n confirming: | 4527 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAATAATGTCGAAGCTCA |
| Suggested primer 2: | AAAGCAACTGGGTGGATGTC |