| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128200 |
| Chromosome: | chromosome_9 |
| Location: | 276964 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCATTGCGTGTTACCTACACAAGGGGGCT |
| Internal bar code: | AAATCTAGTGGGTTTCAGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 99.8395 |
| LEAP-Seq n confirming: | 3111 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCAACGTCACCTACA |
| Suggested primer 2: | TCAGCAGGCTACATGCATTC |