| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.128207 |
| Chromosome: | chromosome 3 |
| Location: | 4058784 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172500 | PTO2,PTOX2 | (1 of 2) K17893 - ubiquinol oxidase (AOX1, AOX2); Plastid terminal oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTAAGCTACCTCGCTTCACCAGTATTG |
| Internal bar code: | GAGTTCGAGTAAATTGCTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 95.0311 |
| LEAP-Seq n confirming: | 153 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAACACCCAGCAATACCG |
| Suggested primer 2: | AGTGATAAGCCCGTGTGGAC |