Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.128213 |
Chromosome: | chromosome 12 |
Location: | 2482530 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g506650 | (1 of 1) PF07064 - RIC1 (RIC1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCACGTATGCGCCAACCTGTACGCAAT |
Internal bar code: | GTCTAGTCCCGCTGCTAAAACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 274 |
LEAP-Seq percent confirming: | 93.3333 |
LEAP-Seq n confirming: | 322 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTACCCCAAGAGCCACC |
Suggested primer 2: | CTGAGGTGGTGGTCAGTGTG |