Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.128221 |
Chromosome: | chromosome_13 |
Location: | 3734591 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589350 | FBB6 | Flagellar/basal body protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCTTGGATCTTCAAGACGCACTGTAATAA |
Internal bar code: | TGACGTATGCATGTGCTTGGCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 386 |
LEAP-Seq percent confirming: | 98.8304 |
LEAP-Seq n confirming: | 169 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAGACGGAGTTAGTGCCTC |
Suggested primer 2: | GAGGTAGCTGAACTCCACCG |