| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.128233 |
| Chromosome: | chromosome 9 |
| Location: | 1213537 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399914 | SSA8 | cilia-sensing, structure and/or assembly; (1 of 1) K10766 - alkylated DNA repair protein alkB homolog 4 (ALKBH4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGCGATCAACGGTCAGGCGAGCCTATT |
| Internal bar code: | TCTTTGATTCTTCATACATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 99.7445 |
| LEAP-Seq n confirming: | 3123 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCCTGCTGTATCACTGT |
| Suggested primer 2: | CACCAGCCTCAGACTTGTCA |