Insertion junction: LMJ.RY0402.128233_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.128233
Chromosome:chromosome_9
Location:1213537
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g399914 SSA8 sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GTAAGCGATCAACGGTCAGGCGAGCCTATT
Internal bar code:TCTTTGATTCTTCATACATCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1007
LEAP-Seq percent confirming:99.7445
LEAP-Seq n confirming:3123
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCCTGCTGTATCACTGT
Suggested primer 2:CACCAGCCTCAGACTTGTCA