| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128235 |
| Chromosome: | chromosome 16 |
| Location: | 431956 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693202 | SIR1 | Ferredoxin-sulfite reductase; (1 of 2) 1.8.7.1 - Assimilatory sulfite reductase (ferredoxin) / Sulfite reductase (ferredoxin) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCGCGTAACGTAAAGCAATTGCCGTAT |
| Internal bar code: | GTGGCCTCCTTGGGGCTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 99.7691 |
| LEAP-Seq n confirming: | 864 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGATGACGGAGCTGAACA |
| Suggested primer 2: | GAAGGGGGAGGAGAATGAAG |