| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.128250 |
| Chromosome: | chromosome 3 |
| Location: | 379602 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144847 | PYK4 | (1 of 2) PTHR11817//PTHR11817:SF3 - PYRUVATE KINASE // PYRUVATE KINASE; Pyruvate kinase 4 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCATCGGCGAGCGGGTTTGCCATACCG |
| Internal bar code: | TGCAGTTAGTCTCGGGCGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 261 |
| LEAP-Seq percent confirming: | 99.611 |
| LEAP-Seq n confirming: | 6914 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCAAGCTTCCATGTCCC |
| Suggested primer 2: | TACCATACTTGCCTGCTCCC |