| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128251 |
| Chromosome: | chromosome 14 |
| Location: | 2307533 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623439 | (1 of 1) 3.4.19.3 - Pyroglutamyl-peptidase I / Pyrrolidone-carboxylate peptidase | 3'UTR|intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGATCATCTGCCTACGAGGCCCTTGCAA |
| Internal bar code: | AAGATCAGGTCTTAATCTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 99.4848 |
| LEAP-Seq n confirming: | 2317 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACTAGACCGACCCACCAA |
| Suggested primer 2: | TGGATGGTGTTTTGCACACT |