| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128253 |
| Chromosome: | chromosome 17 |
| Location: | 1077385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703850 | DC1,ODA3,ODA-DC1,DCC1 | Outer Arm Dynein Docking Complex 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATTGTGTGCGGAGTTTGTGTGGGGCC |
| Internal bar code: | CGTGACATGACCTGGATGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 98.2826 |
| LEAP-Seq n confirming: | 2747 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAACTCAATCACCCCTC |
| Suggested primer 2: | AACACCATTGATGACGAGCA |