| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128273 |
| Chromosome: | chromosome 6 |
| Location: | 2784278 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271100 | CDKG1 | Cyclin dependent kinase; (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAAATCACCCTAGCAATGCCTGGGATGG |
| Internal bar code: | GTGGGCTCGAAAAGGGGTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 99.5868 |
| LEAP-Seq n confirming: | 241 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACAAGCGGGCATTTCAAC |
| Suggested primer 2: | GATCAACACACCGTAGCCCT |