| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128274 |
| Chromosome: | chromosome 2 |
| Location: | 5185553 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105600 | EYE3 | Eyespot assembly protein, ABC1 kinase family; (1 of 2) PTHR10566//PTHR10566:SF45 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAGCAGGCGAAGGGACCCCACCACAA |
| Internal bar code: | CATGATTGTCGCGACGGAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.5633 |
| LEAP-Seq n confirming: | 684 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCGTGTGTGTTTGCTGGA |
| Suggested primer 2: | CCAAAGTCCAGGATGACGAT |