| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128284 |
| Chromosome: | chromosome 12 |
| Location: | 4190688 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518550 | FAP54,C1d-HC1 | Flagellar central pair-associated protein 54; (1 of 1) PF14858 - Domain of unknown function (DUF4486) (DUF4486) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACACACTGCTGGGGCCATACCAGGTG |
| Internal bar code: | TCGACGGGTGAGGCAAAGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 99.9028 |
| LEAP-Seq n confirming: | 1028 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACGAGGTTGGTGTGCCT |
| Suggested primer 2: | CATCGTGTCCAAATCGTGTC |