| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128294 |
| Chromosome: | chromosome_7 |
| Location: | 2497385 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g329500 | SUB1 | Secreted protease and protease inhibitor | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTGGAGTGCAAGGTGTCCAGGCCGATGGA |
| Internal bar code: | CCAAGCCGCACTTGAGGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 99.4511 |
| LEAP-Seq n confirming: | 906 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCGTAGATGAAGTTAGC |
| Suggested primer 2: | TTTTACCCTCCACCTGTTGC |