| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128312 |
| Chromosome: | chromosome 7 |
| Location: | 6122458 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355650 | AMT1F,AMT6 | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGTTCGGGGTTAGCGGGCGAAAGGCAG |
| Internal bar code: | GTGGGATATTTAATTGTCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 99.8898 |
| LEAP-Seq n confirming: | 1813 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAACACCCCTCTTCACC |
| Suggested primer 2: | TTTCCACTTTCCCACTACGG |