Insertion junction: LMJ.RY0402.128318_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.128318
Chromosome:chromosome 10
Location:847511
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g423650 PRPL11 Chloroplast ribosomal protein L11; (1 of 1) PTHR11661//PTHR11661:SF7 - 60S RIBOSOMAL PROTEIN L12 // 50S RIBOSOMAL PROTEIN L11, CHLOROPLASTIC-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCAACACTTGGTGGGCCACTGTCCCGGG
Internal bar code:GATCGGCAGGGACGCCAGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:410
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1168
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGAGTCTGGGTGACTGT
Suggested primer 2:CGAGCACCCCTCTGAGTTAG