| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.128323 |
| Chromosome: | chromosome 12 |
| Location: | 6876799 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g561050 | ELG18,ELG19 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 18 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGGAAGCGCCCCACCGATCGCCCCACC |
| Internal bar code: | TTTTCTTCCGTTCAGGTTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGTACCCAACCAAAGCG |
| Suggested primer 2: | ACAGGTGTACCAGCCGAGAG |