Insertion junction: LMJ.RY0402.128323_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.128323
Chromosome:chromosome 12
Location:6876802
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g561050 ELG18,ELG19 (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 18 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCAGGCATGACTACCGTGTAGGTACCTG
Internal bar code:CCCACAGGACGGTGTTTGGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:766
LEAP-Seq percent confirming:99.7545
LEAP-Seq n confirming:2844
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TTAGGTACCCAACCAAAGCG
Suggested primer 2:ACAGGTGTACCAGCCGAGAG